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X0550-100 – L-Glutamine 100X, 200mM


L-Glutamine 100X, 200mM

  • Sterility : Sterile filtered
  • State : Frozen
  • Storage : -20°C
  • Shelf Life : 24 months
  • Shipping conditions : -20°C or dry ice

Glutamine

  • L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.
  • L-Glutamine is very stable as a dry powder and as a frozen solution.
  • However, in liquid media or stock solutions, L-Glutamine can degrade relatively rapidly.
  • L-Glutamine is also more labile in cell culture solution than other amino acids.
  • Dipeptide derivatives of L-Glutamine (Stable Glutamine) prevent the intramolecular cyclization reaction associated with solutions of L-Glutamine. These derivatives are therefore stable in solution and allow the formulation of cell culture media containing L-Glutamine that may be stored at 4°C for extended periods. Solutions containing these derivatives can even be autoclaved without appreciable degradation of the product (30 minutes at 121°C results in <5% loss of the product).
    The dipeptide derivatives are metabolized within the cells to yield L-Glutamine plus the second amino acid. This results in a more consistent delivery of L-Glutamine to cells and avoids toxic build-up of ammonia in cell cultures.
  • This feature can be especially important for ammonia-sensitive cell lines.
SKU: X0550-100 Categories: , , Tags: ,

Technical Specifications

Description L-Glutamine 100X, 200mM
Volume (ml) 100