, ,

P0191-N10L – IMDM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes


IMDM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes

  • Sterility : Non sterile
  • State : Powder
  • Storage : +2/+8°C
  • Shelf Life : 36 months
  • Shipping conditions : Ambient
  • Addition Sodium Bicarbonate powder : 3.024 g/l
  • Addition Sodium Bicarbonate 7.5% solution : 40.3 ml/l
  • Addition Sodium Bicarbonate 7.5% solution : 40.3 ml/l

IMDM – ISCOVE’S MODIFIED DULBECCO’S MEDIUM

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.

Iscove’s Medium is a variation of Dulbecco’s Modified Eagle’s Medium (DMEM) containing:

  • Selenium, additional amino acids and vitamins
  • Sodium pyruvate
  • HEPES buffer
  • Potassium nitrate instead of ferric nitrate.

Further studies demonstrated that Iscove’s Medium would support:

  • Murine B lymphocytes
  • Hematopoietic tissue from bone marrow
  • B cells stimulated with lipopolysaccharide
  • T lymphocytes
  • a variety of hybrid cells.

Technical Specifications

Description IMDM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes
Volume (Liter) 10