P0191-N1L – IMDM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes

IMDM – ISCOVE’S MODIFIED DULBECCO’S MEDIUM

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.

Iscove’s Medium is a variation of Dulbecco’s Modified Eagle’s Medium (DMEM) containing:

• Selenium, additional amino acids and vitamins
• Sodium pyruvate
• HEPES buffer
• Potassium nitrate instead of ferric nitrate.

Further studies demonstrated that Iscove’s Medium would support:

• Murine B lymphocytes
• Hematopoietic tissue from bone marrow
• B cells stimulated with lipopolysaccharide
• T lymphocytes
• a variety of hybrid cells.