ThinCert™ Cell Culture Inserts 6 Well, 12 Well & 24 Well

• Stable clear polystyrene housing • Hanging geometry • Sealed PET capillary pore membrane • Single, sterile blister packing • Improved cell adhesion through physical surface treatment • Simplified pipetting due to self-lift geometry • Enhanced pipetting access and gas exchange.

6 Well ThinCert™

Cat.-No.
657 640
657 641
657 610
657 630
657 631
657 638
Pore size [μm]
0.4
0.4
1.0
3.0
3.0
8.0
Pore density [cm-2]
1 x 108
2 x 106
2 x 106
0.6 x 106
2 x 106
0.15 x 106
Optical membrane properties
translucent
translucent
translucent
translucent
translucent
translucent
Culture surface [mm2]
452.4
452.4
452.4
452.4
452.4
452.4
Height (overall) [mm]
16.25
16.25
16.25
16.25
16.25
16.25
Inner/Outer diameter (top) [mm]
24.85 / 27.85
24.85 / 27.85
24.85 / 27.85
24.85 / 27.85
24.85 / 27.85
24.85 / 27.85
Working vol. ThinCert™ [ml]
1.0 – 3.6
1.0 – 3.6
1.0 – 3.6
1.0 – 3.6
1.0 – 3.6
1.0 – 3.6
Working vol. well [ml]
2.0 – 4.15
2.0 – 4.15
2.0 – 4.15
2.0 – 4.15
2.0 – 4.15
2.0 – 4.15
TC surface treatment/Sterile
+ / +
+ / +
+ / +
+ / +
+ / +
+ / +
Multiwell plates per box
4
4
4
4
4
4
ThinCert™ inserts per box
24
24
24
24
24
24
Price/Each (Rs.)
620.00
620.00
620.00
620.00
620.00
620.00
12 Well ThinCert™
Cat.-No.
665 640
665 641
665 610
665 630
665 631
665 638
Pore size [μm]
0.4
0.4
1.0
3.0
3.0
8.0
Pore density [cm-2]
1 x 108
2 x 106
2 x 106
0.6 x 106
2 x 106
0.15 x 106
Optical membrane properties
translucent
translucent
translucent
translucent
translucent
translucent
Culture surface [mm2]
113.1
113.1
113.1
113.1
113.1
113.1
Height (overall) [mm]
16.25
16.25
16.25
16.25
16.25
16.25
Inner/Outer diameter (top) [mm]
13.85 / 15.85
13.85 / 15.85
13.85 / 15.85
13.85 / 15.85
13.85 / 15.85
13.85 / 15.85
Working vol. ThinCert™ [ml]
0.3 – 1.0
0.3 – 1.0
0.3 – 1.0
0.3 – 1.0
0.3 – 1.0
0.3 – 1.0
Working vol. well [ml]
1.0 – 2.0
1.0 – 2.0
1.0 – 2.0
1.0 – 2.0
1.0 – 2.0
1.0 – 2.0
TC surface treatment/Sterile
+ / +
+ / +
+ / +
+ / +
+ / +
+ / +
Multiwell plates per box
4
4
4
4
4
4
ThinCert™ inserts per box
48
48
48
48
48
48
Price/Each (Rs.)
540.00
540.00
540.00
540.00
540.00
540.00
24 Well ThinCerts™
Cat.-No.
662 640
662 641
662 610
662 630
662 631
662 638
Pore size [μm]
0.4
0.4
1.0
3.0
3.0
8.0
Pore density [cm-2]
1 x 108
2 x 106
2 x 106
0.6 x 106
2 x 106
0.15 x 106
Optical membrane properties
translucent
translucent
translucent
translucent
translucent
translucent
Culture surface [mm2]
33.6
33.6
33.6
33.6
33.6
33.6
Height (overall) [mm]
16.25
16.25
16.25
16.25
16.25
16.25
Inner/Outer diameter (top) [mm]
8.4 / 10.4
8.4 / 10.4
8.4 / 10.4
8.4 / 10.4
8.4 / 10.4
8.4 / 10.4
Working vol. ThinCert™ [ml]
0.1 – 0.35
0.1 – 0.35
0.1 – 0.35
0.1 – 0.35
0.1 – 0.35
0.1 – 0.35
Working vol. well [ml]
0.4 – 1.2
0.4 – 1.2
0.4 – 1.2
0.4 – 1.2
0.4 – 1.2
0.4 – 1.2
TC surface treatment/Sterile
+ / +
+ / +
+ / +
+ / +
+ / +
+ / +
Multiwell plates per box
2
2
2
2
2
2
ThinCert™ inserts per box
48
48
48
48
48
48
Price/Each (Rs.)
540.00
540.00
540.00
540.00
540.00
540.00

ThinCert™Plate

The in-vitro reconstruction of many types of epithelia, such as skin, cornea and airway epithelium, requires that the cells used differentiate at the air-liquid-interphase (air-lift culture). Therefore, the tissue is cultivated in permeable membrane supports (cell culture inserts), with the cell culture medium nourishing the cells from below the membrane and the air exerting its influence from above. If cell culture inserts and conventional cell culture plates are utilised in the air-lift culture, the available volume of culture medium is severely limited. This reduced volume results in an elevated nutrient consumption rate from the lower rate compartment and an increased number of medium exchanges. With the novel ThinCert™ plate, Greiner Bio-One offers an innovative solution for the air-lift culture with ThinCert™ cell culture inserts. The plate is available in 6 and 12 well format. Its deep wells allow a larger volume of medium to be available to the air-lift culture. As a consequence, the frequency of medium exchanges may be reduced to one medium change per week. Furthermore, the reduced frequency of medium changes ensures that the medium conditioning lasts longer and an improved tissue quality is achieved. The ThinCert™Plate design consists of notches for fixing the position of the inserted ThinCert™ cell culture inserts. This ensures a stable and predictable position of the inserts during the entire process of cell culture. With ThinCert™ Cell culture inserts and the optimised ThinCert™ Plate Greiner Bio-One provides the professional answer to the challenges of tissue reconstruction in vitro.
 
Reconstruction of a full thickness skin in Vitro.
a)
A permeable membrane support carries fibroblasts in an extracellular matrix (e.g. Collagen). This dermis equivalent is cultivated under submersed culture conditions. b) Keratinocytes are superimposed upon the dermis equivalent and differentiated at the air-liquid-interphase (air-lift-culture). If performed in a regular cell culture plate only a very limited medium volume is available for the air-lift culture. c) The ThinCert™ Plate enables the air-lift culture to access an increased medium volume thereby reducing the frequency of medium exchanges. This allows the cells to stay longer in their conditioned environment which may lead to an increase in tissue quality.
ThinCert™Plate Cell Culture Multiwell Plates

• Optimised for use with ThinCert™ cell culture inserts • Deep wells for an increased volume of medium in air lift culture • Available in 6 and 12 well format. • Notches for fixed insert position.


ThinCert™ Plate: Notches (arrows) secure the position of the ThinCert™ cell
culture inserts during cell culture.
 
 
Cat.-No.
657 110
665 110
Well format
6 well
12 well
Total volume per well [ml]
30
10
Working volume in air-lift culture [ml]
20
4
Sterile
+
+
Lid
+*)
+*)
Quantity per bag/case
1/50
1/60
Price/Each (Rs.)
2200.00
2200.00
*) With condensation rings.