The irrigating buffers and salt solutions are sterile physiological balanced solutions intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. They were used for the first steps for preparing parts of organs and during the dissociation process or for isolation of cell suspensions. Further applications are the intermediate steps for cultivation of cells like washing, centrifugation, suspending and counting and also many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells only for minutes or maximal some hours into suspension. These buffers and solutions are no cell culture media.

They are composed of a Phosphate buffer system, sodium chloride to adjust the osmolarity and perhaps sugar for a short time nutrition and stabilisation of morphology. For applications where Ca²+ and Mg²+ ions interfere with enzyme activity e.g. Trypsin, use the modified buffers w/o Calcium w/o Magnesium.

DULBECCO’S PHOSPHATE BUFFERED SALINE (DPBS)
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0615-100   Dulbecco’s Phosphate Buffered Saline w/o Calcium w/o Magnesium     100 ml   625.00
L0615-500   Dulbecco’s Phosphate Buffered Saline w/o Calcium w/o Magnesium     500 ml   1000.00
L0615-1000   Dulbecco’s Phosphate Buffered Saline w/o Calcium w/o Magnesium     1000 ml   1800.00
L0625-500   Dulbecco’s Phosphate Buffered Saline w/ Calcium w/ Magnesium     500 ml   1150.00
L0625-1000   Dulbecco’s Phosphate Buffered Saline w/ Calcium w/ Magnesium     1000 ml   2000.00
X0515-100   Dulbecco’s Phosphate Buffered Saline 10X w/o Calcium w/o Magnesium     100 ml   880.00
X0515-500   Dulbecco’s Phosphate Buffered Saline 10X w/o Calcium w/o Magnesium     500 ml   1400.00
X0520-500   Dulbecco’s Phosphate Buffered Saline 10X w/o Calcium w/o Magnesium     500 ml   1900.00
EARLE’S BALANCED SALT SOLUTIONS (EBSS)
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0601-500   Earle’s Balanced Salts Solution w/o Calcium w/o Magnesium     500 ml   1150.00
L0602-500   Earle’s Balanced Salts Solution w/ Calcium w/ Magnesium     500 ml   1900.00
X0112-500   Earle’s Balanced Salts 10X w/ Calcium w/ Magnesium w/o Sodium Bicarbonate     500 ml   1950.00
X0113-500   Earle’s Balanced Salts 10X w/o Calcium w/o Magnesium w/o Sodium Bicarbonate     500 ml   2450.00
HANKS’ BALANCED SALT SOLUTIONS (HBSS)
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0605-500   HBSS w/o Ca w/o Mg w/o Sodium Bicarbonate w/o Phenol Red     500 ml   1800.00
L0606-500   HBSS w/ Calcium w/ Magnesium w/ Sodium Bicarbonate w/ Phenol Red     500 ml   1150.00
L0607-500   HBSS w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/o Phenol Red     500 ml   1300.00
L0608-500   HBSS w/ Calcium w/ Magnesium w/o Sodium Bicarbonate w/ Phenol Red     500 ml   1950.00
L0611-500   HBSS w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/ Phenol Red     500 ml   1300.00
L0612-500   HBSS w/ Calcium w/ Magnesium w/ Sodium Bicarbonate w/o Phenol Red     500 ml   1400.00
X0507-500   HBSS 10X w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/o Phenol Red     500 ml   1900.00
X0509-500   HBSS 10X w/ Calcium w/ Magnesium w/o Sodium Bicarbonate w/ Phenol Red     500 ml   1900.00
X0510-500   HBSS 10X w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/o Phenol Red     500 ml   1900.00
X0513-500   HBSS 10X w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/ Phenol Red     500 ml   1900.00
HEPES
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0180-100   HEPES Buffer 1 M     100 ml   2700.00
L0180-500   HEPES Buffer 1 M     500 ml   9800.00
OTHER SALT SOLUTIONS & SALTS
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0640-500   Sodium Chloride Salt Solution 0.85%     500 ml   1450.00
L0642-100   Sodium Pyruvate 100 mM     100 ml   1750.00
L0642-500   Sodium Pyruvate 100 mM     500 ml   2400.00
L0643-100   Potassium Cholride 0.075 M     100 ml   760.00
L0643-500   Potassium Cholride 0.075 M     500 ml   1700.00
L0630-100   Versene     100 ml   1950.00
L0680-100   Sodium Bicarbonate 7.5%     100 ml   775.00
Antibiotics
The use of antibiotics is a helpful tool in the cell culture field or where fluids have to be conserved and prevented from bacterial contamination. Most of the antibiotics suppress the growth of micro-organisms by blocking an anabolic pathway.
NANOMYCOPULITINE actively kills the bacteria in all three stages of development without interfering with the eukaryotic metabolism.
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0008-020   Kanamycin Solution 100X     20 ml   5100.00
L0008-100   Kanamycin Solution 100X     100 ml   13500.00
L0009-050   Amphotericin B     50 ml   4300.00
L0009-100   Amphotericin B     100 ml   4300.00
L0010-020   Antibiotic-Antimycotic 100X     20 ml   2100.00
L0010-100   Antibiotic-Antimycotic 100X     100 ml   6000.00
L0011-010   Gentamicin Sulfate 10 mg/ml     10 ml   2750.00
L0011-100   Gentamicin Sulfate 10 mg/ml     100 ml   16500.00
L0012-010   Gentamicin Sulfate 50 mg/ml     10 ml   9800.00
L0012-100   Gentamicin Sulfate 50 mg/ml     100 ml   58500.00
Penicillin-Streptomycin
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0014-100   Glutamine-Penicillin-Streptoycin 100x     100 ml   6500.00
L0015-010   G-418 (Geneticin) Solution     10 ml   9900.00
L0015-100   G-418 (Geneticin) Solution     100 ml   54250.00
L0018-020   Penicillin-Streptomycin     20 ml   On request
L0018-100   Penicillin-Streptomycin     100 ml   2050.00
L0022-020   Penicillin-Streptomycin Solution100X     20 ml   800.00
L0022-100   Penicillin-Streptomycin Solution100X     100 ml   2000.00
L-X16-010   Nanomycopulitine Concentrat 20 x     10 ml   6850.00
L-X16-100   Nanomycopulitine Concentrat 20 x     100 ml   24950.00