BASAL MEDIUM EAGLE (BME)

Basal Medium Eagle is principaly used for diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0042-500   BME w/Earle’s Salts w/o L-Glutamine     500 ml   1150.00
L0044-500   BME w/Earle’s Salts w/o L-Glutamine w/25 mM Hepes     500 ml   1500.00
L0045-500   BME w/Earle’s Salts w/ L-Glutamine w/25 mM Hepes     500 ml   on request
L0046-500   BME w/Hanks’ Salts w/o L-Glutamine     500 ml   1400.00
CMRL 1066
CMRL was originally formulated for promoting «L» Earl cells growth.
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0053-500   CMRL 1066 w/o L- Glutamine     500 ml   4500.00

DULBECCO’S MODIFIED EAGLE MEDIUM
Dulbecco’s Modified Eagle Medium (DMEM) is for supporting and maintening a broad spectrum of mammalian cell types

DMEM High Glucose

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0100-500   DMEM High Glucose w/o L-Glutamine w/25 mM Hepes     500 ml   1600.00
L0101-500   DMEM High Glucose w/o L-Glutamine w/o Sodium Pyruvate     500 ml   1150.00
L0102-500   DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate     500 ml   1300.00
L0103-500   DMEM High Glucose w/Stable Glutamine w/ Sodium Pyruvate     500 ml   1250.00
L0104-500   DMEM High Glucose w/ L-Glutamine w/ Sodium Pyruvate     500 ml   1250.00
L0106-500   DMEM High Glucose w/o L-Glutamine w/ Sodium Pyruvate     500 ml   1150.00
L0107-500   DMEM High Glucose w/Stable Glutamine w/ 25mM Hepes     500 ml   1500.00
DMEM low Glucose
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0060-500   DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate     500 ml   1250.00
L0064-500   DMEM Low Glucose w/o L-Glutamine w/ Sodium Pyruvate     500 ml   1150.00
L0065-500   DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate w/ 25 mM Hepes     500 ml   1350.00
L0066-500   DMEM Low Glucose w/ Stable Glutamine w/ Sodium Pyruvate     500 ml   1250.00
DMEM - Ham’s F12

Studies to determine the nutritional requirements of many cells have been in progress since Eagle’s first reports. The major essential nutrients were identified and work became focused on the media requirements of individual cell types. Many media designed for these purposes are now available.

Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM medium and Ham’s F12 medium, known as DMEM Ham’s F12.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0090-500   DMEM-F12 w/o L- Glutamine w/o Hepes     500 ml   1350.00
L0091-500   DMEM-F12 w/o L-Glutamine w/o Hepes w/o Glucose     500 ml   1400.00
L0092-500   DMEM-F12 w/ Stable Glutamine w 15 mM Hepes     500 ml   1400.00
L0093-500   DMEM-F12 w/ L-Glutamine w/ 15 mM Hepes     500 ml   1400.00
L0094-500   DMEM-F12 w/o L-Glutamine w/ 15 mM Hepes     500 ml   1400.00
L0095-500   DMEM-F12 w/ L-Glutamine w/ 25 mM Hepes     500 ml   1600.00
L0096-500   DMEM-F12 w/o L-Glutamine w/ 25 mM Hepes     500 ml   1600.00
GLASGOW MEM BHK 21
GMEM was originally formulated for BHK 21 cells growing. It is intended for use with adherent cell lines.
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0221-500   GMEM BHK 21 w/L-Glutamine w/o Tryptose Phosphate Broth     500 ml   5250.00
L0222-500   GMEM BHK 21 w/L-Glutamine w/o Tryptose Phosphate Broth     500 ml   4200.00
HAM’S F10 (F-10 NUTRIENT MEDIUM)

To support the growth of Chinese Hamster Ovary Cells under serum free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cells lines.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0130-500   Ham’s F10 w/L-Glutamine w/ 25 mM Hepes     500 ml   1400.00
L0140-500   Ham’s F10 w/ L-Glutamine     500 ml   1300.00
L0145-500   Ham’s F10 w/o L-Glutamine     500 ml   1300.00
               
HAM’S F12 (F-12 NUTRIENT MEDIUM)

Ham’s F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cell and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Cat #   Description     Vol.   Price/Each (Rs.)
L0135-500   Ham’s F12 w/L-Glutamine     500 ml   1350.00
L0136-500   Ham’s F12 w/o L-Glutamine     500 ml   1350.00

HAM’S F14 (F-14 NUTRIENT MEDIUM)

Ham’s F14 medium was developed from the Ham’s F12 which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham’s F14 contains a double concentration of amino acids compared to the Ham’s F12. This product is also supplemented in Calcium chloride and Ascorbic Acid.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0138-500   Ham’s F14 w/6g/L- glucose w/1mg/L ATP     500 ml   5500.00
ISCOVE’S MODIFIED DULBECCO’S MEDIUM (IMDM)

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reducedserum medium supplemented with albumin, transferrin, Iecithin, and selenium. Iscove’s medium is a modification of Dulbecco’s Modified Eagle’s Medium (DME) containing selenium,additional amino acids and vitamins, sodium pryuvate, HEPES buffer, and potassium nitrate instead at ferric nitrate. Further studies demonstrated that Iscove’s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B Cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0190-500   IMDM w/ L-Glutamine w/ 25 mM Hepes     500 ml   1475.00
L0191-500   IMDM w/ stable Glutamine w/ 25 mM Hepes     500 ml   1475.00
L0192-500   IMDM w/o L-Glutamine w/o Hepes     500 ml   1300.00
               
LEIBOVITZ L-15 MEDIUM

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in Co2. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. when properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0300-500   Leibovitz L15 Medium w/o L-Glutamine     500 ml   2350.00
MC COY’S 5A MEDIUM

Mc Coy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy’s 5A media support the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0210-500   McCoy’s 5A w/ L-Glutamine     500 ml   2350.00
L0211-500   McCoy’s 5A w/o L-Glutamine     500 ml   2350.00
MEDIUM 199

A longterm cultivation of cells required addition of a serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0330-500   Medium 199 w/ Hanks’ Salt w/L-Glutamine     500 ml   1600.00
L0355-500   Medium 199 w/ Earle’s Mod. Salts w/L-Glutamine w/1.25g/ I Sodium Bicarbonate     500 ml   1500.00
MEDIUM 199
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0356-500   Medium 199 w/ Earle’s Salts w/o L-Glutamine     500 ml   1650.00
L0360-500   Medium 199 w/ Ealre’s Salts w/L-Glutamine w/25 mM Hepes     500 ml   2350.00
               
MINIMUM ESSENTIAL MEDIA (MEM)

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle’s Balanced Salts, which make them suitable for use in atmospheres charged with CO² gas.

MEM w/Earle’s Salts
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0415-500   MEM w/ Earle’s Salts w/ L-Glutamine     500 ml   1300.00
L0416-500   MEM w/ Earle’s Salts w/ Stable Glutamine     500 ml   1300.00
L0430-500   MEM w/ Earle’s Salts w/o L-Glutamine w/ NEAA     500 ml   1400.00
L0440-500   MEM w/ Earle’s Salts w/o L-Glutamine     500 ml   1150.00
L0444-500   MEM w/ Earle’s Salts w/ L-Glutamine w/ 25 mM Hepes     500 ml   1600.00
L0445-500   MEM w/ Earle’s Salts w/o L-Glutamine w/ 25 mM Hepes     500 ml   1450.00
MEM w/Hanks’ Salts
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0465-500   MEM w/ Hanks’ Salts Solution w/o L-Glutamine     500 ml   1600.00
L0470-500   MEM w/ Hanks’ Salts w/o L-Glutamine w/ 25 mM Hepes     500 ml   2100.00
MEM Alpha Modification
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0475-500   MEM Alpha w/ L-Glutamine w/o Ribonucleosides w/o Deoxyribonucleosides     500 ml   1500.00
L0476-500   MEM Alpha w/o L-Glutamine w/o Ribonucleosides w/o Deoxyribonucleosides     500 ml   1500.00
RPMI 1640 MEDIUM

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukemia cells.

Cat #   Description     Vol.   Price/Each (Rs.)
L0500-100   RPMI 1640 w/ L-Glutamine     100 ml   500.00
L0500-500   RPMI 1640 w/ L-Glutamine     500 ml   1150.00
L0500-1000   RPMI 1640 w/ L-Glutamine     1000 ml   1500.00
L0501-100   RPMI 1640 w/o L-Glutamine     100 ml   500.00
L0501-500   RPMI 1640 w/o L-Glutamine     500 ml   1050.00
L0501-1000   RPMI 1640 w/o L-Glutamine     1000 ml   1500.00
L0498-500   RPMI 1640 w/ stable Glutamine     500 ml   1150.00
L0495-500   RPMI 1640 w/ L-Glutamine w/ 25 mM Hepes     500 ml   1300.00
L0496-500   RPMI 1640 w/ Stable Glutamine w/ 25 mM Hepes     500 ml   1300.00
L0490-500   RPMI 1640 w/o L-Glutamine w/ 25 mM Hepes     500 ml   1400.00
L0492-500   RPMI 1640 Dutch Modification w/o L- Glutamine w/1g/1Na Bicarbonate w/20 mM Hepes     500 ml   1700.00
L0503-500   RPMI 1640 w/o L-Glutamine w/o Folic Acid     500 ml   1950.00
L0505-500   RPMI 1640 w/o L-Glutamine w/o Phenol Red     500 ml   1350.00
SCHNEIDER’S DROSOPHILA MEDIUM
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0207-500   Schneider’s Drosophila Medium     500 ml   2500.00
SERUM REDUCED MEDIA- MCDB

These media were formulated as a reduced serum supplemented medium for the cultivation of human microvascular endothelials cells. For other microvascular cells it is recommended to use it with growth factors like EGF or hormons like hydorcortisone.

Cat #   Description     Vol.   Price/Each (Rs.)
L1200-500   MCDB 105     500 ml   On request
L1202-500   MCDB 131     500 ml   On request
L1203-500   MCDB 151     500 ml   On request
L1205-500   MCDB 201     500 ml   On request
L1206-500   MCDB 302     500 ml   On request