TRYPSIN

Trypsin is a porcine pancreas-dervied enzyme that is commonly used for the dissociation and disaggregation of anchoragedependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0909-100   Trypsin 0.25% in PBS w/o Calcium w/o Magnesium w/ Phenol Red     100 ml   1600.00
L0910-100   Trypsin 0.25% in PBS w/o Calcium w/o Magnesium w/o Phenol Red     100 ml   1725.00
X0915-100   Trypsin 2.5% in PBS w/o Calcium w/o Magnesium w/o Phenol Red     100 ml   1950.00
L0931-100   Trypsin 0.25% EDTA in HBSS w/o Calcium w/o Magnesium w/ Phenol Red     100 ml   1950.00
 
TRYPSIN
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0931-100   Trypsin 0.25% EDTA in HBSS w/o Calcium w/o Magnesium w/ Phenol Red     100 ml   1950.00
L0931-500   Trypsin 0.25% EDTA in HBSS w/o Calcium w/o Magnesium w/ Phenol Red     500 ml   6200.00
L0932-100   Trypsin 0.25% EDTA 0.02% in HBSS w/o Calcium w/o Magnesium w/ Phenol Red     100 ml   1850.00
X0920-100   Trypsin 2.5% in HBSS w/o Calcium w/o Magnesium w/o Phenol Red     100 ml   1600.00
L0930-100   Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/ Phenol Red     100 ml   1450.00
L0930-500   Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/ Phenol Red     500 ml   5900.00
L0940-100   Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/o Phenol Red     100 ml   1450.00
L0940-500   Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/o Phenol Red     500 ml   5900.00
X0930-100   Trypsin-EDTA 10X     100 ml   1750.00
MEM 50X/100X and BME 100X CONCENTRATE AMINO ACIDS, VITAMINS, NEAA

The solutions of amino acids, vitamins or non essential amino acids concentrated can be added to your basal medium (the final concentration of the MEM or BME must be 1X) to enrich it and then this medium is used as a classical MEM or BME.

Cat #   Description     Vol.   Price/Bottle (Rs.)
X0552-100   BME Amino Acids 100X w/o L-Glutamine     100 ml   3500.00
X0554-100   MEM Amino Acids 50X w/o L-Glutamine     100 ml   2100.00
X0556-100   MEM Vitamins 100X w/o L-Glutamine     100 ml   2100.00
X0557-100   MEM non Essential Amino Acids 100X w/o L-Glutamine     100 ml   1800.00
LYMPHOSEP

Lymphosep is designed for the simple, rapid isolation of lymphocytes from whole blood that has been diluted and treated with anti-coagulant or defi-brinating agent.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0560-100   Lymphosep, Lymphocyte Separation Media     100 ml   2550.00
L0560-500   Lymphosep, Lymphocyte Separation Media     500 ml   10500.00
GLUTAMINE

L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution.

However, in liquid media or stock solutions, L-Glutamine can degrade relatively rapidly. L-Glutamine is also labile in cell culture solution than other amino acids.

Dipeptide derivatives of L-Glutamine (Stable Glutamine) prevent the intramolecular cyclization reaction associated with solutions of L-Glutamine. These derivatives are therefore stable in solution and allow the formulation of cell culture media containing L-Glutamine that may be stored at 4°C for extended periods. Solutions containing these derivatives can be even autoclaved without appreciable degradation of the product. (30 minutes at 121° C results in <5% loss of the product).

The dipeptide derivatives are metabolized within the cells to yield L-Glutamine plus the second amino acid. This results in more consistent delivery of L-Glutamine to your cells and avoid toxic build-up of ammonia in your cell cultures. This feature can be especially important for ammonia-sensitive cell lines.

Cat #   Description     Vol.   Price/Bottle (Rs.)
X0550-100   L-Glutamine 100x, 200mM     100 ml   1150.00
X0551-100   Glutamine stable 100x, 200mM     100 ml   2100.00
CELL CULTURE WATER
Cat #   Description     Vol.   Price/Bottle (Rs.)
L0970-100   Cell Culture Water Pyrogen free     100 ml   500.00
L0970-500   Cell Culture Water Pyrogen free     500 ml   1150.00
L0970-1000   Cell Culture Water Pyrogen free     1000 ml   2100.00
COLCEMID

Colcemid arrested the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0040-010   Colcemid 10 μg/ml in PBS (Demecolcin)     10 ml   1600.00
L0040-020   Colcemid 10 μg/ml in PBS (Demecolcin)     20 ml   2900.00
L0040-050   Colcemid 10 μg/ml in PBS (Demecolcin)     50 ml   5500.00
PHENOL RED

Phenol red is used as a pH indicator. The sodium salt of phenol red is used widely in culture media to identify changes from
neutral to acidic pH values. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and
above.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0541-100   Phenol Red Solution 0.5%     100 ml   8800.00
TRYPAN BLUE

Trypan Blue is a stain used to distinguish viable from non viable cells.

Cat #   Description     Vol.   Price/Bottle (Rs.)
L0990-100   Trypan Blue 0.5% Solution (non Sterile)     100 ml   1450.00